02193nas a2200193   4500008004100000022001400041245010900055210006900164300001200233490000700245520151100252653001901763653001001782653001401792653003001806100001701836700001701853856012901870       2006                        eng d  a1055-790300aPhylogenetic relationships and convergence of helicosporous fungi inferred from ribosomal DNA sequences.0 aPhylogenetic relationships and convergence of helicosporous fung  a587-5970 v393 a<p>Helicosporous fungi form elegant, coiled, and multicellular mitotic spores (conidia). In this paper, we investigate the phylogenetic relationships among helicosporous fungi in the asexual genera Helicoma, Helicomyces, Helicosporium, Helicodendron, Helicoon, and in the sexual genus Tubeufia (Tubeufiaceae, Dothideomycetes, and Ascomycota). We generated ribosomal small subunit and partial large subunit sequences from 39 fungal cultures. These and related sequences from GenBank were analyzed using parsimony, likelihood, and Bayesian analysis. Results showed that helicosporous species arose convergently from six lineages of fungi in the Ascomycota. The Tubeufiaceae s. str. formed a strongly supported monophyletic lineage comprising most species from Helicoma, Helicomyces, and Helicosporium. However, within the Tubeufiaceae, none of the asexual genera were monophyletic. Traditional generic characters, such as whether conidiophores were conspicuous or reduced, the thickness of the conidial filament, and whether or not conidia were hygroscopic, were more useful for species delimitation than for predicting higher level relationships. In spite of their distinctive, barrel-shaped spores, Helicoon species were polyphyletic and had evolved in different ascomycete orders. Helicodendron appeared to be polyphyletic although most representatives occurred within Leotiomycetes. We speculate that some of the convergent spore forms may represent adaptation to dispersal in aquatic environments.</p>
10aDNA, Ribosomal10aFungi10aPhylogeny10aPolymerase Chain Reaction1 aTsui, C.K.M.1 aBerbee, M.L.  uhttps://fungi.myspecies.info/content/phylogenetic-relationships-and-convergence-helicosporous-fungi-inferred-ribosomal-dna-001659nas a2200193   4500008004100000022001400041245005500055210005400110300001200164490000800176520108900184653001001273653001101283653003101294100001601325700001601341700002101357856008701378       2009                        eng d  a1469-813700aHow to know unknown fungi: the role of a herbarium0 aHow to know unknown fungi the role of a herbarium  a719-7240 v1813 a<p>The development of a universal approach to the identification of fungi from the environment is impeded by the limited number and narrow phylogenetic range of the named internal transcribed spacer DNA sequences available on GenBank. The goal here was to assess the potential impact of systematic DNA sequencing from a fungal herbarium collection. DNA sequences were generated from a diverse set of 279 specimens deposited at the fungal herbarium of the Royal Botanic Gardens at Kew (UK) and bioinformatic analyses were used to study their overlap with the public database. It is estimated that c. 70% of the herbarium taxonomic diversity is not yet represented in GenBank and that a further c. 10% of our sequences match solely to &#39;environmental samples&#39; or fungi otherwise unidentified. Here it is shown that the unsampled diversity residing in fungal herbaria can substantially enlarge the coverage of GenBank&#39;s fully identified sequence pool to ameliorate the problem of environmental unknowns and to aid in the detection of truly novel fungi by molecular data.</p>
10aFungi10aPlants10aSequence Analysis, Protein1 aBrock, P.M.1 aDöring, H.1 aBidartondo, M.I.  uhttps://spiral.imperial.ac.uk/bitstream/10044/1/4248/1/Brock%20et%20al.%202009.pdf