01908nas a2200157   4500008004100000022001400041245012700055210006900182300001200251490000800263520129200271100001801563700001601581700002601597856012701623       2014                        eng d  a1878-614600aEvolution of ketosynthase domains of polyketide synthase genes in the Cladonia chlorophaea species complex (Cladoniaceae).0 aEvolution of ketosynthase domains of polyketide synthase genes i  a896-9090 v1183 a<p>Lichen-forming fungi synthesize a diversity of polyketides, but only a few non-reducing polyketide synthase (PKS) genes from a lichen-forming fungus have been linked with a specific polyketide. While it is a challenge to link the large number of PKS paralogs in fungi with specific products, it might be expected that the PKS paralogs from closely related species would be similar because of recent evolutionary divergence. The objectives of this study were to reconstruct a PKS gene phylogeny of the Cladonia chlorophaea species complex based on the ketosynthase domain, a species phylogeny of the complex, and to explore the presence of PKS gene paralogs among members of the species complex. DNA was isolated from 51 individuals of C. chlorophaea and allies to screen for the presence of 13 PKS paralogs. A 128 sequence PKS gene phylogeny using deduced amino acid sequences estimated from the 13 PKS paralogs and sequences subjected to BLASTx comparisons showed losses of each of two PKS domains (reducing and methylation). This research provided insight into the evolution of PKS genes in the C. chlorophaea group, species evolution in the group, and it identified potential directions for further investigation of polyketide synthesis in the C. chlorophaea species complex.</p>
1 aTimsina, B.A.1 aHausner, G.1 aPiercey-Normore, M.D.  uhttps://fungi.myspecies.info/content/evolution-ketosynthase-domains-polyketide-synthase-genes-cladonia-chlorophaea-species